Biosynthesis, Chemical Structure, and Structure-Activity Relationship of Orfamide Lipopeptides Produced by Pseudomonas protegens and Related Species.
Identifieur interne : 000C99 ( Main/Exploration ); précédent : 000C98; suivant : 000D00Biosynthesis, Chemical Structure, and Structure-Activity Relationship of Orfamide Lipopeptides Produced by Pseudomonas protegens and Related Species.
Auteurs : Zongwang Ma [Belgique] ; Niels Geudens [Belgique] ; Nam P. Kieu [Belgique] ; Davy Sinnaeve [Belgique] ; Marc Ongena [Belgique] ; José C. Martins [Belgique] ; Monica Höfte [Belgique]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2016.
Abstract
Orfamide-type cyclic lipopeptides (CLPs) are biosurfactants produced by Pseudomonas and involved in lysis of oomycete zoospores, biocontrol of Rhizoctonia and insecticidal activity against aphids. In this study, we compared the biosynthesis, structural diversity, in vitro and in planta activities of orfamides produced by rhizosphere-derived Pseudomonas protegens and related Pseudomonas species. Genetic characterization together with chemical identification revealed that the main orfamide compound produced by the P. protegens group is orfamide A, while the related strains Pseudomonas sp. CMR5c and CMR12a produce orfamide B. Comparison of orfamide fingerprints led to the discovery of two new orfamide homologs (orfamide F and orfamide G) in Pseudomonas sp. CMR5c. The structures of these two CLPs were determined by nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. Mutagenesis and complementation showed that orfamides determine the swarming motility of parental Pseudomonas sp. strain CMR5c and their production was regulated by luxR type regulators. Orfamide A and orfamide B differ only in the identity of a single amino acid, while orfamide B and orfamide G share the same amino acid sequence but differ in length of the fatty acid part. The biological activities of orfamide A, orfamide B, and orfamide G were compared in further bioassays. The three compounds were equally active against Magnaporthe oryzae on rice, against Rhizoctonia solani AG 4-HGI in in vitro assays, and caused zoospore lysis of Phytophthora and Pythium. Furthermore, we could show that orfamides decrease blast severity in rice plants by blocking appressorium formation in M. oryzae. Taken all together, our study shows that orfamides produced by P. protegens and related species have potential in biological control of a broad spectrum of fungal plant pathogens.
DOI: 10.3389/fmicb.2016.00382
PubMed: 27065956
PubMed Central: PMC4811929
Affiliations:
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Kieu</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Crop Protection, Faculty of Bioscience Engineering, Ghent University Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Phytopathology, Crop Protection, Faculty of Bioscience Engineering, Ghent University Ghent</wicri:regionArea><wicri:noRegion>Ghent University Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Sinnaeve, Davy" sort="Sinnaeve, Davy" uniqKey="Sinnaeve D" first="Davy" last="Sinnaeve">Davy Sinnaeve</name><affiliation wicri:level="1"><nlm:affiliation>NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Ghent University Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Ghent University Ghent</wicri:regionArea><wicri:noRegion>Ghent University Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Ongena, Marc" sort="Ongena, Marc" uniqKey="Ongena M" first="Marc" last="Ongena">Marc Ongena</name><affiliation wicri:level="1"><nlm:affiliation>Microbial Processes and Interactions Unit, Faculty of Gembloux Agro-Bio Tech, University of Liège Gembloux, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Microbial Processes and Interactions Unit, Faculty of Gembloux Agro-Bio Tech, University of Liège Gembloux</wicri:regionArea><wicri:noRegion>University of Liège Gembloux</wicri:noRegion></affiliation></author><author><name sortKey="Martins, Jose C" sort="Martins, Jose C" uniqKey="Martins J" first="José C" last="Martins">José C. Martins</name><affiliation wicri:level="1"><nlm:affiliation>NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Ghent University Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Ghent University Ghent</wicri:regionArea><wicri:noRegion>Ghent University Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Hofte, Monica" sort="Hofte, Monica" uniqKey="Hofte M" first="Monica" last="Höfte">Monica Höfte</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Crop Protection, Faculty of Bioscience Engineering, Ghent University Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Phytopathology, Crop Protection, Faculty of Bioscience Engineering, Ghent University Ghent</wicri:regionArea><wicri:noRegion>Ghent University Ghent</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Orfamide-type cyclic lipopeptides (CLPs) are biosurfactants produced by Pseudomonas and involved in lysis of oomycete zoospores, biocontrol of Rhizoctonia and insecticidal activity against aphids. In this study, we compared the biosynthesis, structural diversity, in vitro and in planta activities of orfamides produced by rhizosphere-derived Pseudomonas protegens and related Pseudomonas species. Genetic characterization together with chemical identification revealed that the main orfamide compound produced by the P. protegens group is orfamide A, while the related strains Pseudomonas sp. CMR5c and CMR12a produce orfamide B. Comparison of orfamide fingerprints led to the discovery of two new orfamide homologs (orfamide F and orfamide G) in Pseudomonas sp. CMR5c. The structures of these two CLPs were determined by nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. Mutagenesis and complementation showed that orfamides determine the swarming motility of parental Pseudomonas sp. strain CMR5c and their production was regulated by luxR type regulators. Orfamide A and orfamide B differ only in the identity of a single amino acid, while orfamide B and orfamide G share the same amino acid sequence but differ in length of the fatty acid part. The biological activities of orfamide A, orfamide B, and orfamide G were compared in further bioassays. The three compounds were equally active against Magnaporthe oryzae on rice, against Rhizoctonia solani AG 4-HGI in in vitro assays, and caused zoospore lysis of Phytophthora and Pythium. Furthermore, we could show that orfamides decrease blast severity in rice plants by blocking appressorium formation in M. oryzae. Taken all together, our study shows that orfamides produced by P. protegens and related species have potential in biological control of a broad spectrum of fungal plant pathogens. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">27065956</PMID><DateCompleted><Year>2016</Year><Month>04</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Biosynthesis, Chemical Structure, and Structure-Activity Relationship of Orfamide Lipopeptides Produced by Pseudomonas protegens and Related Species.</ArticleTitle><Pagination><MedlinePgn>382</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2016.00382</ELocationID><Abstract><AbstractText>Orfamide-type cyclic lipopeptides (CLPs) are biosurfactants produced by Pseudomonas and involved in lysis of oomycete zoospores, biocontrol of Rhizoctonia and insecticidal activity against aphids. In this study, we compared the biosynthesis, structural diversity, in vitro and in planta activities of orfamides produced by rhizosphere-derived Pseudomonas protegens and related Pseudomonas species. Genetic characterization together with chemical identification revealed that the main orfamide compound produced by the P. protegens group is orfamide A, while the related strains Pseudomonas sp. CMR5c and CMR12a produce orfamide B. Comparison of orfamide fingerprints led to the discovery of two new orfamide homologs (orfamide F and orfamide G) in Pseudomonas sp. CMR5c. The structures of these two CLPs were determined by nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. Mutagenesis and complementation showed that orfamides determine the swarming motility of parental Pseudomonas sp. strain CMR5c and their production was regulated by luxR type regulators. Orfamide A and orfamide B differ only in the identity of a single amino acid, while orfamide B and orfamide G share the same amino acid sequence but differ in length of the fatty acid part. The biological activities of orfamide A, orfamide B, and orfamide G were compared in further bioassays. The three compounds were equally active against Magnaporthe oryzae on rice, against Rhizoctonia solani AG 4-HGI in in vitro assays, and caused zoospore lysis of Phytophthora and Pythium. Furthermore, we could show that orfamides decrease blast severity in rice plants by blocking appressorium formation in M. oryzae. Taken all together, our study shows that orfamides produced by P. protegens and related species have potential in biological control of a broad spectrum of fungal plant pathogens. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Zongwang</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Crop Protection, Faculty of Bioscience Engineering, Ghent University Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Geudens</LastName><ForeName>Niels</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Ghent University Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kieu</LastName><ForeName>Nam P</ForeName><Initials>NP</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Crop Protection, Faculty of Bioscience Engineering, Ghent University Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sinnaeve</LastName><ForeName>Davy</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Ghent University Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ongena</LastName><ForeName>Marc</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Microbial Processes and Interactions Unit, Faculty of Gembloux Agro-Bio Tech, University of Liège Gembloux, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martins</LastName><ForeName>José C</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Ghent University Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Höfte</LastName><ForeName>Monica</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Crop Protection, Faculty of Bioscience Engineering, Ghent University Ghent, Belgium.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Magnaporthe oryzae</Keyword><Keyword MajorTopicYN="N">P. protegens CHA0 and Pf-5</Keyword><Keyword MajorTopicYN="N">biocontrol</Keyword><Keyword MajorTopicYN="N">fluorescent pseudomonads</Keyword><Keyword MajorTopicYN="N">nonribosomal peptide 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first="Nam P" last="Kieu">Nam P. Kieu</name><name sortKey="Martins, Jose C" sort="Martins, Jose C" uniqKey="Martins J" first="José C" last="Martins">José C. Martins</name><name sortKey="Ongena, Marc" sort="Ongena, Marc" uniqKey="Ongena M" first="Marc" last="Ongena">Marc Ongena</name><name sortKey="Sinnaeve, Davy" sort="Sinnaeve, Davy" uniqKey="Sinnaeve D" first="Davy" last="Sinnaeve">Davy Sinnaeve</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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